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Image Search Results
Journal: Science Advances
Article Title: Spermine and spermidine bind CXCR4 and inhibit CXCR4- but not CCR5-tropic HIV-1 infection
doi: 10.1126/sciadv.adf8251
Figure Lengend Snippet: ( A ) Identification of chromatographic fractions (from Source 15RPC) of an SP-derived compound library that inhibit X4-HIV-1 but not R5-HIV-1 infection. Fractions were added to TZM-bl cells and infected with X4-HIV-1 or R5-HIV-1. X4-HIV-1–inhibiting fractions 15 to 18 were shown to contain spermine and spermidine (see fig. S1). ( B ) TLC plate image showing separation of dansyl-derivatized (see fig. S2A) fractions (two pooled per lane) in cyclohexane:ethyl acetate (2:3, v/v). ( C and D ) Effect of s ynthetic spermine (C) and spermidine (D) on X4-HIV-1 and R5-HIV-1 infection. TZM-bl cells were preincubated for 1 hour with spermine or spermidine and infected with X4-HIV-1 or R5-HIV-1 NL4-3 V3 loop variants. ( E ) Effect of spermine on R5 T/F HIV-1 infection of TZM-bl cells. ( F and G ) Spermine targets a cellular factor involved in X4-HIV-1 entry. TZM-bl cells or X4-HIV-1 were incubated for 1 hour with indicated concentrations of spermine (F) or spermidine (G). Thereafter, cells were infected with either X4-HIV-1 (cell treatment) or virions/polyamine mixtures and inoculated on TZM-bl cells, thereby diluting spermine 10-fold (virion treatment). ( H and I ) Spermine was added 0 to 60 min before infection with X4-HIV-1 (H) or 2 hours after infection with X4-HIV-1 or R5-HIV-1 (I) of TZM-bl cells. RLU, relative light units. For all experiments, infection rates were measured 2 days after infection by β-galactosidase assay. Values shown represent mean values of one (A, B, E, and H) or three (C, D, F, G, and I) experiments performed in triplicates ± SD.
Article Snippet: The M < 30 kDa filtrate was spiked with 0.1% heptafluorobutyric acid (HFBA) and applied to a
Techniques: Derivative Assay, Infection, Incubation